pH monitoring in bioreactors and cell culture

At ZP we are super interested in many  types of biosensors including pH sensor. At ZP we appreciate that pH sensors have not changed in 40 years, so at ZP we are leading the revolution in updating and upgrading pH sensors both for discreet and continuous  monitoring.


The technology at ZP offers pH sensors at a few cents per sensors when purchased and used in volume.



In this note we describe the continuous monitoring of the pH levels of the media within a bioreactor, specifically we have used E. coli DH5α which has been  cultured in lysogeny broth (LB) for 24-48 hours. During this time the sensor was

recording the potential/pH of the solution by OCP. The sensor was tested for pH sensitivity using the standard pH4, pH7 and pH10 reference solutions at 10 minute intervals both before and after the microbiological test to firstly ensure that the sensor was pH sensitive before the test began, and secondly to assess what impact (if any) the exposure to the

bacterial solution had upon the sensors pH sensitive properties. 2019